Tuza, Zoltán András and Siegal-Gaskins, Dan and Kim, Jongmin and Szederkényi, Gábor (2015) Analysis-based Parameter Estimation of an in vitro Transcription-Translation System. In: ECC 2015 - 14th Annual European Control Conference. EUCA, Linz, pp. 1560-1566. ISBN 978-3-9524269-4-4 10.1109/ECC.2015.7330760

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Abstract

Recent advances in measurement technology pro- vide us with rich source of data for estimating parameters in biomolecular circuit models, particularly in simplified in vitro transcription-translation systems, so-called molecular “bread- boards”. In this paper, we elaborate on a mass action type dynamic model for such an in vitro system and detail a parameter estimation procedure that may be used with time series data containing information about both transcriptional and translational stages of gene expression. The identification process is supported by structural identifiability analysis to ensure proper model structure. Statistical analysis and vali- dation of the estimated parameter set help us to understand the characteristics of point estimation results.

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